Rapid Staining Tetrazolium Test
Full germination tests take a number of days to conduct.
Therefore a quick test that gives a reliable indication of germination levels is required.
The rapid stain tetrazolium test aims to determine the percentage of a sample that has the potential to germinate under optimum conditions.
Tetrazolium is a colourless chemical that reacts with hydrogen in living cells to produce a red colour meaning living tissue can be distinguished from non-living tissue. It only detects tissues that are alive or viable.
A tetrazolium test will not take into account other factors that affect germination i.e. chemical damage, dormancy, disease.
- Cut 50 corns length ways
- Discard one half and place the other half in the slide
- Cover with tetrazolium solution
- Incubate at 40⁰C for 10 minutes and drain
- Count the number of corns with a live embryo and are stained pink
- Multiply the number of live corns by two to get the percentage